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To prevent automated spam submissions leave this field blank.The effect of testosterone on the oestradiol binding component of the androgen receptor in prostatic membrane preparations.
The androgen receptor in the rat prostate has been shown to be a protein with a molecular weight of 65,000-68,000, which binds to androgen in a cooperative manner. This receptor is not occupied by oestrogen, but it is stimulated by oestrogen in vitro. The present study compared the effect of testosterone treatment on the oestradiol binding component of the androgen receptor in four prostatic membrane preparations obtained from intact, castrated and testosterone-treated rats. In each case the binding of [3H]17-alpha-oestradiol to the androgen receptor was inhibited by the addition of either 50 or 100 pmol oestradiol per assay tube. This inhibition of binding could be prevented by the addition of an excess of unlabelled 17-alpha-oestradiol, indicating that it was a specific binding phenomenon. Maximal steroid inhibition was reached with a dose of 100 pmol 17-alpha-oestradiol/assay tube. At a dose of 100 pmol 17-alpha-oestradiol/assay tube the proportion of the binding sites occupied in the presence of oestrogen was higher in the membranes of rats treated with testosterone than in the membranes from the castrates or intact rats. This was demonstrated by the displacement curves obtained with oestradiol, mestranol, testosterone and 5 alpha-dihydrotestosterone. The effect of testosterone on the androgen receptor was most pronounced in the membranes obtained from rats treated with testosterone. The present results show that testosterone directly stimulates the oestradiol-binding component of the androgen receptor and that this androgen-dependent stimulation may have a role in the development of the prostate.Q:

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Waaris (1988) Synopsis : Waaris Is A Bollywood Hindi Family Drama Movie, Directed By Raveendra Peepat, Starring Raj Babbar, Smita Patil, Amrita All (1)IMDb 6.82 h 32 min1988ALL.
Free Download Movie Songs · Movie Name: Waaris – 1988 · Release Date: 0000-00-00 · Movie Category: Not available.
Waaris Is A Bollywood Hindi Family Drama Movie, Directed By Raveendra Peepat, Starring Raj Babbar, Smita Patil, Amrita All Rp  .
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